RNA isolation / purification Tissue
Complete disruption of cells to isolate total RNA from tissues can be challenging. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice.
Start discussionFound 2 discussions for this experiment
Discussion
4 years ago
4 years ago by
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
4 years ago
4 years ago by
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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